Access denied trying to publish

I am using VS 2010 with TFS and I am trying to publish my web application project to our local webserver.  I tried both to our IIS7 and our IIS6 webservers with the same results.  When I click Publish I get the following error for any web page I have changed since the last publish.  This just started happening and not sure what is cause.  Thanks.

------ Publish started: Project: ClinicAccounting, Configuration: Debug Any CPU ------
Connecting to R:\ClinicAccounting...
Transformed Web.config using Web.Debug.config into obj\Debug\TransformWebConfig\transformed\Web.config.
Copying all files to temporary location below for package/publish:
obj\Debug\Package\PackageTmp.
Publishing folder /...
Unable to add 'SearchHome.aspx' to the Web site.  Unable to add file 'SearchHome.aspx'.  Access is denied.

etc...